How can I prevent bubbles from forming under a coverslip?

My colleague and I are having some trouble mounting 70 micrometer mouse brain slices. When we initially position the coverslip over the mounted slices, there are no bubbles but when we come back...

10 February 2021 3,580 1 View

Can anyone suggest why my PCR results gave big differences in the control group?

I did RT-PCR using SYBR Green and used 2 ul of cDNA per sample to investigate the effect of LPS in primary osteoclast culture. I loaded the same amount of RNA to make cDNA (550ng/sample). However...

17 September 2020 9,193 3 View

Does anyone have a recommendation for a book about Raman related to carbon besides Dresselhaus?

I need more than specifically graphene-related raman. I would like a book more related to changes in various carbon-chains. Thank you.

26 August 2020 5,101 3 View

Hi, can anyone clear up about the molarity concept?

I'm required to prepare 2mM of LiCl for my DNA extraction method, which I calculated to be 0.0212 g of LiCl in 250 ml of water. My supervisor told me to use any volume as long as it is 2 mM....

14 February 2020 9,808 3 View

How can I extract road features from an optical image, can someone suggest some algorithms to perform that?

I want to code and process from scratch, by using the available libraries. Which could be the appropriate methods when one have only a single image?

28 March 2018 216 6 View

About online business acceptance journals?

Hi, Can someone please recommend what ISI or Scopus indexed journals that I can submit my manuscript ? my study is about what is people perception/acceptance of online business in asia? whether...

31 January 2018 7,181 7 View

How can we speed up the time of publishing students work, without turn a frustration for their career? Is it getting worse?

Some data suggest that wait times have increased within certain subsets of journals, such as popular open-access ones and some of the most sought-after titles. At Nature, the median review time...

10 February 2016 7,715 9 View

Where can i find Reves (1983) What makes a good language learner?

I'm looking for an often cited work in the field of Foreign language aptitude research, but which doesn't seem to be available (easily) online: Reves. T. (1983). What makes a good language...

01 January 1970 7,165 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View