Hello everyone,
I need to isolate cell membrane of leukocytes and platelet, from frozen buffy coat. Any suggestion?
You can either do subcellular fractionation (http://docs.abcam.com/pdf/protocols/subcellular_fractionation.pdf)
or use a commercial kit.
Any suggestions on how to distinguish between activated and exhausted human lymphocytes T CD8 with flow cytometry? It is known that exhausted lymphocytes T have higher expression of inhibitory...
28 December 2020 255 3 View
Hi, can anyone help me to understand if this conference about phytoremediation is a scum? https://waset.org/phytoremediation-phytotechnologies-and-management-conference-in-july-2020-in-prague...
15 January 2020 8,841 3 View
Hi everyone, I am trying to produce eGFP lentiviral vector by transducting HEK 293T with calcium phosphate method. I transduce cells when they are at 70 % confluent (following this method...
14 October 2018 2,351 4 View
Hi! We are trying to estimate body mass (W) heritability and cross-sex genetic correlation using MCMCglmm. Our data matrix consists of three columns: ID, sex, and W. Body mass data is NOT...
25 July 2017 6,702 1 View
Hello, I'm working on melanoma and I'm searching the best melanoma cell lines (easy to culture) recapitulating all the steps of melanoma growth: Radial growth-phase cell line, vertical...
05 June 2017 8,061 2 View
I wish to stain fibronectin in 3D tumor spheroids made with human cancer cells and fibroblasts. do you have a good antibody to suggest? Have you ever used the references MA5-11981 from...
30 June 2016 1,973 2 View
When choosing a WAPLS model, apart from relying on performance (e.g. r2, r, max bias, RMSEP, scatter-plot etc..), the recommendation is to select the successive component if the model performance...
15 May 2016 1,200 2 View
20 April 2016 3,381 3 View
31 March 2016 9,050 8 View
I performed a multiple choice experiment to assess the food preference of the sea urchin Paracentrotus lividus in relation to 5 different food items. I have 15 replicates, each represented by a...
03 February 2016 8,060 4 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why
02 March 2021 1,388 3 View
02 March 2021 3,060 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?
01 March 2021 8,087 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View