Could anyone identify the artefacts in my cell culture?

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Does anyone know who developed LAPT broth? What does LAPT stand for?

LAPT broth: 1.5% Bacto Peptone, 1% Bacto Tryptone, 1% yeast extract, 1% glucose and 0.1% Tween 80

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What alarm limits do you set for apnea monitoring your neonates in NICU? What are the clinical reasons for choosing that particular alarm settings?

I have met clinicians setting their inferior limit alarm for apnea, in critically ill children, at 5 seconds; cannot understand this because pauses in ventilation of 5 to 10 seconds are normal...

19 July 2023 4,429 2 View

Surgical Pleth Index limitations, when I can and cannot use SPI tu guide intraoperative opioid administration?

influence of ephedrine on Surgical Pleth Index values during anesthesia. Can SPI still be used to monitor NANB when vasoactive drugs are given?

03 July 2023 1,023 2 View

How does the neighborhood approach uncover power dynamics and promote equitable refugee integration?

How does the neighbourhood approach, which examines local contexts and dynamics, help uncover the role of power dynamics and social hierarchies in shaping the social, economic, and political...

05 June 2023 9,437 0 View

Does anyone have experience with inhibitor remover for removing tert-butylcatechol?

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03 January 2023 7,151 1 View

How to permeabilize exosomes to evaluate its cargo through FCM?

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19 June 2022 4,626 0 View

How to permeabilize exosomes to evaluate its cargo through FCM?

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19 June 2022 4,442 0 View

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How can I culture NSC34 properly?

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01 March 2022 2,741 0 View

Are frozen tips necessary for collagen manipulation?

Hello everybody! I have to make some collagen scaffols and I dissolve the collagen in 0,2% acetic acid. My question is: since collagen is sensible to temperature and it can polymerize, is it...

20 February 2022 968 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

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07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

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E.coli contamination in human RNA seq data ?

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04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

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Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Prem Subramaniam

It is a normal feature of the cell line....some cells have odd membrane protrusions, like "tails". See the image of this STS-verified and mycoplasma free image attached. Taken and modified from here: https://cls.shop/TK6/300357

(red lines in attachment and so many other cells)

Robert Adolf Brinzer

Could be invadopodia, lamellipodia, filopodia or microvilli.