Unfortunately, there is no centrifuge in our lab spinning at 20,000 x g so I wondered whether I can just use our centrifuge at Maximum rcf ( 5000 x g) and spin the sample by doubling of the time given in the protocol. E.g. the protocol dictates 20,000 x g for 30 min and I would perform just 5,000 x g for 60 min. Would this lead to the same result or is there maybe a Problem with precipitation in Terms of Plasmid Isolation (Problems with insufficient precipitation of Proteins and chromosomal DNA)? Thanx for any suggestions !