I was wondering if anyone has any experience concerning detection of cytotoxicity of carotenoids, eg crocins and crocetins with alamar blue method.

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Where can I find the number of genomic copies of 16S RNA of Pseudoramibacter Alactolitycus?

Could you please indicate me a source, or any useful information for where can I find the genome copies of 16s RNA of pseudoramibacter alactolyticus. I need this information for several other...

23 March 2023 2,609 1 View

What is the molecular weight of Double standed DNA ????

Hello to all, I need to perform a standard curve for metagenomic analysis with qPCR, of Treponema Denticola and Pseudoramibacter Alactolyticous, using the 16S RNA copies of my DNA. As well I...

17 March 2023 837 3 View

Which bioclimatic indices can I estimate with temperarure (oC) and percipitation (mm) data?

I have temperature and percipitation data for some years and I would like to examine the thermal comfort of the region. In my knoledge even the calculation of the simpliest indices requires...

20 December 2020 7,362 3 View

How do you deal with error in the proccess of tranfering data from Spss to MPlus?

Dear all, I am trying to transfer data from Spss to Mplus. I save the data in Spss in a comma separated format but when I am trying to import it in MPlus I get the following error: * ERROR The...

27 May 2020 4,117 6 View

How can I get individual-level residuals of indicators before SEM?

Hi, I am new to SEM and I have a question regarding individual-level residuals. I would like to analyse survey data using SEM in Stata. I want to include three latent variables in my analysis....

26 March 2019 233 3 View

Bacterial 16S universal primers

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Participants For Research

Hi there! I am a Masters student in Counseling Psychology in City College, University of York and i am looking to recruit participants for my dissertation research. The theme is 'The impact of...

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How can I delete a question from research gate ?

How can I delete a question from research gate

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Black dots or infection

I am performing some primary pulp cells cultures and I have noticed that in my petri dishes I do have some black dots. I use DMEM 10% with antibiotics and antifugal. I apologies for the poor...

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

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How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

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To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

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What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

Can a photocatalytic degradation of methylene blue from red mud be pseudo- zero order kinetics?

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29 July 2024 7,404 1 View

Assistance with MTT Assay Data ?

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26 July 2024 7,511 3 View

Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

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23 July 2024 4,603 4 View

How do we pick data for determination of Validation Acceptance Criteria?

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23 July 2024 7,292 3 View

My question concerns MTT cell viability assay?

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21 July 2024 9,914 1 View