I was preparing the phusion pcr rection mixture for 120 microlitre ...but i mistakenly added 20 microltr less nuclese free water , what will happen ?

Partha Dey @Partha-Dey-12

12 September 2018 0 4K Report

g-c buffer - 24 micro ltr

mgcl2 - 2.4

dNTPs - 2.4

Fp - 2.4

Rp - 2.4

DMSO -3.6

phusion pol- 1.2

g.DNA -7.5

Mili Q H2O -56.5

water should be 76.5 microlitre but i added 56.5...

what will happen to my reaction?

amplification will come or not

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