Partha Dey

4 Questions 2 Answers 0 Followers

Questions related from Partha Dey

Whether annealing temperature of standard PCR changes overtime?

Dear Researcher, I facing a lot of problem in PCR amplification of deletion construct of few budding yeast gene, However the The the reaction set up, annealing temp. (55 degree C) standardized...

25 November 2023 8,465 1 View

Unable to get transformants in MATalpha strain of Saccharomyces cerevisiae, please suggest me why?

Dear yeast biologist, I performed 4-5 time yeast transformation in S. cerevisiae SK1 MATalpha strain but I am unable to get transformants. I performed and checked all the necessary steps as per my...

18 November 2022 4,223 1 View

I am unable to get colonies after 5 yeast transformation in S. cerevisiae SK1 strain background (MATalpha & MATa ), please suggest me why?

Please suggest me why I am not able to get transformants and how can I troubleshoot the problem? Initially when I transformed and selected on Hygromycin and G418 plate, I got 5 to 15 colonies per...

28 October 2022 5,534 3 View

I was preparing the phusion pcr rection mixture for 120 microlitre ...but i mistakenly added 20 microltr less nuclese free water , what will happen ?

g-c buffer - 24 micro ltr mgcl2 - 2.4 dNTPs - 2.4 Fp - 2.4 Rp - 2.4 DMSO -3.6 phusion pol- 1.2 g.DNA -7.5 Mili Q H2O -56.5 water...

12 September 2018 3,515 0 View