I am trying wrapping Ecoli BL21 cell extract into POPC liposomes but they burst in a very short time. However, when I wrap LB in the liposomes, they remain stable for a long time.(Liposomes are in PBS buffer)
We suspect it is because there are still remaining lipases in our BL21 extract and these lipases degrade our POPC membrane. We do not exclude the possibility that osmotic pressure or other components in the cell extract may play a role in our liposomes' burst.
Did you come across similar situations or do you have any ideas about the burst?
Many thanks for any suggestions or hypothesis!
Dear Sun
It may happen due to a high amount of cholesterol in your liposome formulation if you used it.
BOC Sciences has many years of experience in liposome research and can help you perform liposome characterization and analysis, and solve the problems you encounter in liposome research.
https://liposomes.bocchem.org/
The content of your BL21 cell extract may play a role here, but you can first evaluate the ingredients and proportions of your liposome formulation and literature studies. What is your liposome formulation and proportions. Since adding cholesterol makes the structure more stable, it is a desired liposome component to be added.
- Badges
- Science topic
- Similar topics
- Engineering
- Chemical Engineering
- Membranes