Go for Paraffin sectioning. Prefer 4% Paraformaldehyde as a fixative instead of 10% formalin. To avoid auto fluorescence Antigen retrieval must be done.
http://www.ihcworld.com/epitope_retrieval.htm
Dear Sachin, may I suggest reading the following RG-thread @ http://www.researchgate.net/topic/Histology/post/Is_there_any_suitable_staining_method_protocol_for_lipids_in_paraffin_sections?
as well as: https://www.researchgate.net/post/Does_anyone_have_ideas_about_lipid_fixation_for_fat_staining_in_paraffin_sections
Perhaps you'll find there some valuable information on your task. More detailed information about the proteins and lipids you want to localize (in which tissue?) would be an advantage/beneficial for helping you more efficiently. Regards, Wolfgang
Dear Kumar.
You could embedded the biological specimen in glycol methacrylate and perform the desirable histochemistry protocols.
Check out the following publication: http://www.ncbi.nlm.nih.gov/pubmed/14680922
Good work
Paraffin cut;
Pearse's Method for phospholipids. (Luna, 1968)
Landing's Method for Lipids (Luna, 1968).
Mucosubstans as protein.
Alcian Blue Ph 2.5, 1.0, 0.4
aldehyde fucsin, PAS, Aldehyde fucsin/Alcian blue etc.
Several protocols are available for histochemistry without cryostat section
but always keep in mind that if you are using the samples fixed very long back may encounter problems with antigen retrieval.
So better to give minimum fixation time.
What kind antigen you want to locate in birds.
If you need any help you can contact me @ [email protected]
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