Hi,
I want to isolate exosomes for RNA analysis from blood serum. Blood samples take about 5 hours to reach the laboratory. İn which conditions can I transport blood samples properly????
Mustafa Bey, Just make sure that you do not freeze the blood. You can carry it at +4 to +8 safely. Perhaps you can put an ice pack at the bottom of your box, then put some packing on it and then blood ( to make sure that the blood container does not sit on the ice directly), then more packing material on the top. However, bear in mind that more extracellular vesicles can be produced during the transport. It means that the EV content of your blood might differ from the fresh blood.
Do you want to get RNA from whole blood? Then it should not be a problem. But consider that you will most likely have emolisis and that many circulating RNAs (not associated to your vesicles) will be found free in that suspension. In order to separate the vesicles from contaminating RNAs the best technique that you can use is chromatography and then use some hemolisis controls (microRNas). Finally, at least once, you should check that in your exosome pellet you don't have Ago2, since it is a protein often found circulating and associated to microRNAs.
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