Non-light sensitive cellular stains?

Hello everyone, I want to stain live and dead cells in a fresh tissue. There are a large variety of stains (i.e, PI, cell tracker, FDA, DAPI). As long as I know, the should not be exposed to...

09 October 2018 6,571 1 View

How can I measure the surface roughness of a live tissue?

Hello everyone, I need to know the surface roughness of fresh tumor tissue. Has anybody ever done that? Is it just similar to measuring the surface roughness of a glass or metal surface?

08 September 2018 9,364 3 View

Is it possible to culture cryostat slices?

I need to make flat slices of a tumor tissue and I need to keep the tissue alive. I have access to a vibratome and a cryostat. Can anyone tell me which one is better and why? Is it possible to...

07 August 2018 5,434 2 View

Cell laboratory contamination

I'm working in an animal cell laboratory(on human cancer cells). it's about 2 weeks that all kinds of cells get infected and die a day after defreezing. I have disinfected the laboratory and...

05 June 2017 2,484 16 View

What are the differences among photoresists?

04 May 2017 8,028 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View