11 November 2014 2 3K Report

staining way

More Peng Bao-yu's questions See All
Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

Mass spectra averaging algorithm?

I am now developing a python module for ms2 database searching, would like to realize a function that similar to what Xcalibur did, choose multiple mass spectra and get an averaged spectra. But...

22 July 2024 3,975 1 View

Concentration of gelatin as brain model ?

I am using gelatin to model mechanical properties of brain tissues in-vitro. Do you have any suggested concentration ? Thank you so much.

19 July 2024 3,095 0 View

Is the protecting group boc of the amino group stable at 37°C?

I have a small molecule reagent with a boc-protected amino group. Now the reaction needs to be reacted at 37°C for 30 h. Is this protection group stable?

12 July 2024 3,745 2 View

How to obtain authoritative data on the international shipping industry?

It is best to have the route distance between ports, the size of the shipping alliance market, direct service, the number of ships owned by the shipping alliance and the congestion of ships in the...

06 July 2024 845 1 View

Vissim "lane closure" works well on "first" link, but not working on "second" link?

I attempted to model a temporary 'lane closure' due to an accident or work zone. Initially, I successfully closed Lane 1 on Link 1, and it worked as expected. However, when I tried to close Lane 1...

17 June 2024 2,070 0 View

What are the parameters for simulating plastic deformation cracks in concrete using the CDP model?

Dear professors and colleagues， hello！Recently, while studying the CDP model, I have read the manuscripts of various experts and have gained a lot, but I still have some questions.What I...

10 June 2024 1,906 1 View

What factors affect the real-time volume during the simulation in Vissim?

I was trying to model a freeway entrance in Vissim do some "variable speed limit" comparison experiments. in "scenario 1", I set up the Vehicle Input with 8 time...

04 June 2024 9,053 1 View

Low-yield of Cas9 plasmid after maxi-preparation. Anyone has the same issues?

Hi~ We are making different Cas9 plasmids such as regular spCas9, base editing and prime editing under different promoter such as CMV and CAG. During selection of colonies, we always get a good...

22 May 2024 7,336 0 View

Your question needs a question mark?

When I calculated Kd, I found that although the concentrations were set to satisfy the type of binding used to determine the differential spectrum, Kd was unstable if the two high concentrations...

14 May 2024 399 0 View

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Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

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How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

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Senescence-associated beta galactosidase staining is False Positive in control group?

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Suggestions for the Annexin V Positive Control ?

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