Yoshida's (Y) and Hoagland's (H) are in my mind. Literatures say that both nitrate as well as ammoniacal forms of nitrogen are essential in order to maintain a balance in the pH. So If I use Y my purpose is solved as both the forms are provided by Ammonium nitrate. But Y is an older version of nutrient solution, however I prefer it. If I use H I'll be loosing the ammoniacal form as I won't be adding Ammonium dihydrogen phosphate coz I need a phosphorus less solution. But my guide prefers me using H. Plese suggest me a better one.
Partiendo de la solución nutritiva (sn) de Hoagland, y cuando lo que se pretende es reducir o eliminar el suministro de P (suministrado normalmente en forma de KH2PO4), normalmente también se reduce o elimina el suministro de K, lo que obliga a complementar el defecto de K por otras vías. Cualquiera de las dos alternativas más habituales lleva al mismo resultado:
1.- En forma de KOH, lo cual no es aconsejable pues implicaría un aumento del pH, que finalmente tendría que corregirse aportando algún ácido. El PO4H3 quedaría excluido, el NO3H aportaría NO3-, y el SO4H2 aportaría SO42-. Teniendo en cuenta que un pequeño aumento de la concentración SO42- en la solución nutritiva no tiene por que afectar al cultivo de modo significativo, esta última sería quizás la opción mas aceptable.
2.- En forma de KNO3 o de K2SO4. Dado que emplear KNO3 no es aconsejable por lo dicho anteriormente, quedaría utilizar K2SO4 que, al igual que en el caso anterior, implicaría un pequeño aumento de la concentración de SO42- en la solución nutritiva, que por otro lado, no tiene porque afectar al cultivo de modo significativo.
En mi opinión, y por lo que puedo entender de tu pregunta, creo que lo procedente en este caso ería suministrar el defecto total o parcial de K en forma de K2SO4.
Please consider using a much more dilute medium than Hoagland's solution and replacing it more often. If the pH drifts between replacements it is easily adjusted.
Does your research question simply require P deficient plants?
I have in the past used a modified Hoagland's for P-deficient experiment and worked well. Depending on the duration of your experiment, you should change your solution periodically and consider beginning with a quarter strength solution, followed by half strength and then full strength solution. At the beginning and whenever the nutrient solution is replaced, check and adjust the pH to your requirement.
@Everyone: Thank You!! N Sorry for replying too late. Over the time I've convinced myself to go with Yoshida's Solution (Chapter17 http://books.irri.org/9711040352_content.pdf) for the reason mentioned above in my query and (@Mr. Valsa Remony Manoj) to play safe in order to escape from adverse reactions of the element; in the name of standardizing my own nutrient solution. @Paul Milham: 'P' starvation is required for Expressional (RNA) studies and Root structural studies. @Michel P. Abu : Yes.. I'll take care of the pH @ 5. @Miguel Corbella: However, I've thought of replacing Ammonium nitrate and Potassium sulphate with Ammonium sulphate and Potassium nitrate @ a later stage.
Question:
I've planned to grow three wheat plant for 24 days with 400 mL of the nutrient solution for a week and then it'll be replaced. For the concentration of various element please refer the above. Is this sufficient??
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