I will need the probe specification that can transmit through the cranium and pick the ventricles at different level. An average depth from dorsal to ventral surface of the rat brain is less than 2 cm. Any previous experience/paper in Ultrasonographic assessment of rat brain ventricles will be great.
A 12-15 MHz probe should do the trick, but for adult animals, you will need to thin the skull; also, having a stereotaxic frame will help with precision. This article link may also help:https://www.ncbi.nlm.nih.gov › NCBI › Literature › PubMed Central (PMC)
Thanks, The article link is incomplete. How do you thin the skull of a living animal, I tried scanning with a 10mHz linear probe, but it wasnt going through the cranium.
In this paper they explain how to thin the skull.
1. Neuroimage. 2016 Jan 1;124(Pt A):752-61. doi: 10.1016/j.neuroimage.2015.09.037. Epub 2015 Sep 28.
Transcranial functional ultrasound imaging of the brain using microbubble-enhanced ultrasensitive Doppler.
Errico C1, Osmanski BF1, Pezet S2, Couture O1, Lenkei Z2, Tanter M3
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