06 April 2022 3 10K Report

Carbon emission data for 35 to 40 years in West Bengal

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Does avidin binds to streptavidin?

Does avidin binds to streptavidin?

14 April 2024 5,692 1 View

I'm having problem with intense background noise in immunofluorescence for my protein of interest conjugated with streptavidin in mouse liver?

I'm having a problem with intense background noise in immunofluorescence for my biotinylated protein of interest conjugated with streptavidin (attached with a fluorophore) in mouse liver tissue...

11 April 2024 6,395 0 View

Why do I experience random death of cells after passaging?

Hello Everyone, I am culturing few different kinds of adherent cells. However, I sometimes experience cells dying in the flask after passaging them in one flask whereas same cells suspension...

07 February 2024 4,354 0 View

How to trypsinize IMR90 cells to get good viability?

I have a question regarding culturing fibroblast IMR90. I use DMEM+10%fbs+1% pen/strep for culturing them. I use 0.05% trypsin EDTA for trypsinization for 40 sec in the incubator and add DMEM +...

18 January 2024 958 1 View

IMR90 cell viability and immunoflorescence for alpha SMA staining?

I have a question regarding culturing fibroblast IMR90. I use DMEM+10%fbs+1% pen/strep for culturing them. I use 0.05% trypsin EDTA for trypsinization for 40 sec in the incubator and add DMEM +...

20 December 2023 4,363 0 View

What should be the final conc of PI in apoptosis/necrosis assay with annexin V in flow cytometry?

What should be the final conc of PI used?

11 October 2023 587 2 View

What is the mechanism behind biological nitrogen fixation in green microalgae in axenic medium ? without any bacterial association ?

I want to know about the mechanism through which biological nitrogen fixation occurs in photosynthetic green microalgae apart from previously reported bacterial associations. Like in axenic lab...

11 August 2023 3,256 2 View

How does one can distinguish between Donor-acceptor level transition and free to bound by blueshift and redshift in laser dependent PL spectrum study?

I have got redshift in PL spectra for one material and blueshift in another material with increasing excitation power. I want to interpret the DAP and and free to bound transition clearly. Can...

06 July 2023 3,819 0 View

Can I culture suspension cell line (Jurkat) in flasks used for adherent cell line?

In our lab we have cell culture wares for adherent cell lines which are coated. Can I use the same flask for culturing suspension cell line (Jurkat)?

25 June 2023 4,412 2 View

NetCDF files having the extension .nc4, into CSV file or geotiff file?

My goal is to prepare a script in python or R to work with netCDF files having the extension .nc4, example Daily GPM (Global Precipitation Measurement) precipitation data (Late Run) into CSV or...

08 April 2023 7,659 2 View

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Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

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Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

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Who of all of you will help TPEOM to re-originate the Earths atmosphere to it original 5 atmospheric elements/gases?

Global ‘Weather’ has stopped, as ‘aqua thermionic instituted by the ‘Global thermo atmospherics has instituted global thermodynamics of 120º C + and thus all waters are, or have boiled into a...

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Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

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How can I separate graphene from amorphous carbon of similar particle sizes?

The starting material is a finely ground powder. Some particles contain more graphene than others.

30 July 2024 3,978 2 View

Question about combustion Kinetics ?

I am currently working on modeling FCC catalyst regeneration and have come across a point of uncertainty that I hope you can assist me with. In my previous models, I have utilized lumped kinetics...

30 July 2024 6,137 0 View

What are the perfect mathematical model for a solar-powered ship implementation?

A computational study of heat flux on cylindrical surfaces using CNT hybrid nanofluids

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Why semicircle of nyquist plot is not complete?

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Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM images?

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