Why we use chloroforme in the preparation of feather meal?
Can we replace it by other substances less dangerous and more available?
How can i prépare a pectine gélose to détect the pectinase activité in bacteria
19 March 2023 4,803 0 View
Hi I need a protocol for screening of gelatinase activity for spring water bacteria How I prepare the gelose and how I use it
05 March 2023 3,762 0 View
Steps How to use nitrocefin to detect bêta lactamase's presence
21 February 2023 3,146 1 View
I wanna to prepare feather meal for my research and I need the protocol for preparation
20 February 2023 7,687 3 View
I'm gonna prepare a nutritive gelose and I want to use it in the same day. It's that possible? If not how long should I wait to use it?
12 February 2023 2,668 1 View
I'm trying to prepare R2B/R2A media with seawater to cultivate marine bacteria after autoclaving (110°C) my media. I systematically observe a important precipitate. Despite many tests (in...
30 June 2021 2,715 3 View
After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...
29 July 2024 9,753 3 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View
Good evening, I would like to evaluate the heavy metal content in bird feathers. Can I keep my samples in powder form ? and can I use other tubes than Teflon for digestion?
04 July 2024 158 4 View
I need to do a Schiff base reaction starting from a ketone and 1,10-phenanthroline-5-amino. I've seen that both of these starting materials are not very soluble in methanol and ethanol, which is...
23 June 2024 5,925 3 View
Is there a competent body to certify and judge whether a new anti-seismic technology makes a positive contribution or whether it is dangerous?
21 June 2024 9,919 3 View
and chloroformate. But it takes 10 days to compete the reaction. How can I shorten the time of the reaction?
14 June 2024 2,924 3 View
Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...
06 June 2024 5,715 4 View
Hi everyone, I'm working on a project related to seismic risk analysis and I am searching for online tools that provide hazard curves and hazard disaggregation for various locations around the...
05 June 2024 851 15 View
Greetings We are trying to study Cholesteryl esters. We start with MeOH/Chloroform extraction and then remove most of the noise by silica column, but the Cholesteryl esters come out with...
02 June 2024 5,545 0 View
We have been trying to extract RNA from brown algae. We have been following traditional CTAB method as modified by Sim et al., 2013. We get a very thick white jelly interface with the Chloroform...
30 May 2024 356 0 View