Screening of gelatinase activity ?

Adouane Ouiam @Adouane-Ouiam

06 March 2023 0 4K Report

I need a protocol for screening of gelatinase activity for spring

water bacteria

How I prepare the gelose and how I use it

Badges
Science topic

More Adouane Ouiam's questions See All

What is the protocole to prépare an gélose aded with pectine?

How can i prépare a pectine gélose to détect the pectinase activité in bacteria

19 March 2023 4,803 0 View

Steps How to use nitrocefin to detect bêta lactamase's presence?

Steps How to use nitrocefin to detect bêta lactamase's presence

21 February 2023 3,146 1 View

What is the simple technique to prepare a feather meal?

I wanna to prepare feather meal for my research and I need the protocol for preparation

20 February 2023 7,687 3 View

I wanna to prepare feather meal at home but I don't have chloroforme Can I replace it by something else?if yes , what is it?

Why we use chloroforme in the preparation of feather meal? Can we replace it by other substances less dangerous and more available?

19 February 2023 1,121 0 View

After preparation of culture medium can it be used on the same day?

I'm gonna prepare a nutritive gelose and I want to use it in the same day. It's that possible? If not how long should I wait to use it?

12 February 2023 2,668 1 View

In Microbiology, how do you avoid R2B/R2A media with seawater to precipitate ?

I'm trying to prepare R2B/R2A media with seawater to cultivate marine bacteria after autoclaving (110°C) my media. I systematically observe a important precipitate. Despite many tests (in...

30 June 2021 2,715 3 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

MAGeCK Plotting Error: Logarithmic Axis Must Have Positive Limits?

I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...

28 July 2024 9,811 1 View

Screening of EPS producer LAB?

if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...

26 July 2024 6,606 0 View

How to extract binding energy from pv.maegz file without using Schrodinger?

I have conducted virtual screening using Schrödinger on a database of 17,000 molecules. Unfortunately, I cannot use the system with the Schrödinger license at the moment. I am trying to find a way...

18 July 2024 2,881 4 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

How to Dedust Activated Carbon Granules?

Dear all, We are producing activated carbon from hazelnut shells but have an issue about the dust of the a.c. granules also known as the dust film on granules. Linear vibrating seives doesn't do...

11 July 2024 9,782 2 View

How can I solve the problem with Autodock 1.5.7 version, Command prompt?

I follow the protocol in this paper "Computational protein–ligand docking and virtual drug screening with the AutoDock suite" for the "Flexible Docking". Everything is smooth until I write the...

22 June 2024 3,089 2 View

Which is more preferred method for the screening of phytochemicals of a plant sample, aqueous or any organic solvents ?

I have tried to screen various phytochemicals of different plants leaves sample with antimicrobial property. I have tried aqueous extract of plants. But i have doubt whether sample is actually...

18 June 2024 8,947 4 View

I only why i only get bands on the membrane after staining but no bands after the antibody-blocking steps?

Hello, I have started working with Western blot recently and have difficulties getting the beta-actin band. I recently tried the steps for getting a beta-actin band from the intestinal sample of...

17 June 2024 3,750 4 View

Please describe clear and elaborate process of separating solids from garbage enzyme/ eco enzyme after 90 days of fermentation.?

How we should screen out the solid material left after 90 days of fermentation in making of garbage enzyme. Is there any standardized process.

17 June 2024 4,840 3 View