I wanna design QPCR probe. i do have primer for the particular gene, i like to design Taqman probe for the primer. Can any one suggest ?

More Vinu S Siva's questions See All

How to simulate a new protocol in VANET Toolbox in MATLAB?

I've downloaded the VANET Toolbox in MATLAB, but there aren't any detailed documentation on how to use it. Can anyone please explain the step by step process in designing the simulation...

01 November 2020 7,262 2 View

Which simulator other than NetSim is easy to build VANET sims? How to implement a MAC protocol and analyse the throughput,transmissions?

I have an assignment where I have to implement a latest protocol in VANET and analyse the results. I have chosen the OCA-MAC a TDMA based MAC protocol. Which simulator is easiest to implement...

01 November 2020 518 3 View

Effect of excess cyclodextrin on drug after cyclodextrin reaches maximum solubility in phase solubility study?

I am working on phase solubility studies between beta-cyclodextrin and drug? I used more concentration of beta-cyclodextrin till 30mM despite its maximum solubility of 16mM. Usually, above maximum...

30 October 2020 3,921 9 View

About Input factors which has chosen for wear examination?

1. Why 5, 10 and 15% of SiC was chosen? any base reference paper related to it. Why not below 5% is to be included. 2. What will be the result if the % of SiC is reduced less than 5% in wear...

27 October 2020 6,635 2 View

Is there a way to implement a large strain viscoelastic material model in AUTODYN?

I am new to AUTODYN and have used a little bit of ANSYS workbench. I have been looking at implementing a visco-hyperelastic constitutive model in AUTODYN. However, I could not find any way to do...

12 October 2020 5,245 3 View

Is a physical theory and its mathematical interpretation describes the same at the most fundamental level?

No. At the most fundamental level, Interpretation of physical theory by mathematics may not be appropriate. The reason- "Suppose a Circle of radius zero and with theta(zero) radians. It is...

21 September 2020 8,124 8 View

Do zif-8 has absorbance?

Do zif-8 have absorbance? And after synthesis and purification, zif-8 needs to be stored in powder or can be dispersed in aqueous or organic solvents

13 August 2020 242 1 View

How to frame the title of the manuscript or research proposal or thesis?

I would like to request the eminent researchers to guide us, which phase of the research the title has to be framed and on what basis? Thanks in Advance

19 May 2020 1,984 11 View

I would like to know if there is anyone who is working on CEP126 gene(also known as KIAA1377)??

As there is very little data available for CEP126 gene on databases, i would like to know if there is anybody or any lab working on this gene.

31 January 2020 1,860 1 View

What is the recommeneded hardness test for weld joint ( Rockwell-A or B or C , Brinell , Vickers).i want recommened answer with proper reason?

what is the recommeneded hardness test for weld joint ( Rockwell-A or B or C , Brinell , Vickers).i want recommened answer with proper reason. The weld sample is 5mm thick steel plate.

03 December 2019 7,043 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View