I wanna design QPCR probe. i do have primer for the particular gene, i like to design Taqman probe for the primer. Can any one suggest ?

More Vinu S Siva's questions See All

I am getting a result that total quantum states for any particle is around 900 only. But as per quantum mechanics they may be infinite. Any comment ?

All the elementary particles are quantum states of photon and photon will have all these 900 quantum states in this nature. If I am right , what is your comment on it? If anybody feel that this...

11 April 2024 7,365 3 View

Is there any relation between a particles rotation(rotation on its own axis)and its quantum spin ?

If a point on its surface(if we suppose it as a sphere with radius 'r')moves with linear velocity 'v'(relativistic speed) and the mass pf that particle is 'm'. How can we calculate its quantum...

11 March 2024 9,837 9 View

How do we characterize the short-, medium-, and long-range-order quantitatively in atomic distances?

Does short-range order (SRO) reflect the one-bond distances, i.e., 1-2 Å? How do we demarcate the medium- and long-range order?

28 November 2023 7,614 1 View

Im not able to see any protein bands.This is how our Nylon membrane looks after taking out it from semidryer.whats the problem?

we are applying 24v for 40mins.After that our nc mebrane is becoming like the image i posted, we tried to change current and timing, every time it is becoming like this.

29 October 2023 9,589 0 View

Why separation of toluene and benzene occurs at 3 bar pressure in a distillation column?

I was asked to design a benzene production plant via a thermal toluene hydrodealkylation process, and one of the parts was to separate the benzene and toluene via distillation. I am unsure why the...

27 October 2023 9,095 1 View

Dear Professors and Researchers. Does anyone know how to install and work in Vienna Ab initio Simulation Package (VASP)?

22 October 2023 428 2 View

How to determine the volume of buffer for peptide purification using D-tube dialyzer?

How to determine the volume of buffer for unconjugated peptide removal from nanocomposite using a D-tube dialyzer, also How to test check whether the free peptide can be removed by this process or...

02 October 2023 8,153 2 View

Is it ADHD self Diagnosable, by the person who feels he/her under going with the ADHD symptoms?

I want to Know is it ADHD is self Diagnosable by the persons who undergoing with the symptoms of ADHD.

15 July 2023 7,290 5 View

We are facing the problem with agarose gel electrophoresis.after completing running the gel half of the agarose is not stained?

Even though we are mixing the ethidium bromide properly before agarose gel casting We are facing this problem of uneven staining.Its not that the staining is random, but exactly half.We tried...

11 May 2023 409 3 View

Why Deciliter unit is used in clinical measurements?

Why the unit- per decilitre is used more often than per ml or per litre in clinical measurements. Is there any historical reasons behind this? Thanks in advance.

02 May 2023 363 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Rui Liu

Hi Vinu, a lot of things are needed to consider such as specificity, Tm value, C+G content, reporter and quencher. I find a website with some suggestions in Chinese, you can try to read it. http://www.bio1000.com/experiment/fenzi/361370.html

Vinu S Siva

Dear Rui, hope you are doing well. Thanks, i try to translate and go through to site you given.