After the first round of PCR to amplify B. coli DNA, there was no detectable band after electrophoresis, I then used the PCR product as the template with the same prime set used in the first reaction, this gave me a very thick and sharp band. But I do not know what to call this method, is it nested or hemi-nested?
When you use either forward or reverse (only one primer) with one previously used primer, then it is called as semi-nested PCR.
Hi Mike, this is just double round of amplification, since you are using the same primers.
I hope this helps.
Hi Mike, as Marcos De Donato said, it is double amplification. To call nested PCR, you should have pair of new primer which binds the amplicons of first PCR (hence, its product size smaller than first PCR) and to call as hemi(semi)-nested PCR, you use one of the first PCR primer along with a new primer.
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