I used PCR product as the template with the primary primers set for second PCR.What name do I give this process? Is it nested or hemi-nested?

After the first round of PCR to amplify B. coli DNA, there was no detectable band after electrophoresis, I then used the PCR product as the template with the same prime set used in the first...

12 December 2015 8,982 5 View