I use 60ng/ul of RNA for cDNA preparation using iSCRIPT kit.....i go for nanodrop for cDNA quantification and found 1ug/ul of cDNA...is this possible?

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Rosanna del Gaudio

it is not clear to me if you did the check, that is if you read the same mixture in the nanodrop before incubation!You did it?

Paul Rutland

The iscript kit will have oligo dT and random primers as well as dNTPs all of which will absorb in the UV so unless you have purified the cDNA you may be reading reagents not product

Liquan Guo

You should consider increasing the RNA concentration or changing the primers