In-house ELISA too much signal. Any suggestions ?

09 November 2020 5,509 5 View

Hi everyone, I am considering to buy pH3000 research grade pH sensor and electrode from Sensorex. Has anybody used that before?

If not, any recs for a good pH sensor to use in the lab for in vitro assays? Thank you so much

20 September 2020 7,416 1 View

Is this journal a serious journal?

Hello everyone, I want to know your opinion about: "journal of musculoskeletal and neuronal interactions" is it a serious and real journal (not a predatory one) to publish in? Thanks

25 July 2020 3,806 4 View

What is the best solvent/method to be use to prepare PVB (Polyvinyl butyral) solution?

I would like to spin-coat a thin film of PVB - the PVB is in powder form - which solvent should I use to prepare the PVB solution and what are the process to be followed? Thanks

04 February 2019 8,677 15 View

Could anyone tell me how to find refractive index of SiO2 thin film, from its UV/Vis transmission spectrum?

I have recorded UV/Vis transmission spectrum of SiO2 thin film deposited on glass. I want to find the refractive index of the film. My transmission spectrum does not represent interference...

01 June 2018 5,500 5 View

Is remote sensing data an alternative of field readings?

The use of remote sensing for the assessment of a aquatic resources provides several advantages compared to conventional approaches for water quality assessments. So, can we replace the field data...

04 February 2018 3,269 31 View

Where do I find data on global mean annual Lake surface temperature?

01 March 2017 977 3 View

Does chemotherapy affect RNA quality and quantity after its extraction from tissues?

15 January 2017 5,413 2 View

How to reconstruct high dimensional space from low dimensional space?

22 March 2016 9,168 3 View

Is there a protocol for seeding 0.5 cell in 96 wells plate?

I have not worked with 96 well plate; I need to split my cells into 96 well plate, seeding 0.5 cell/200 micolit media/ well. How I can plate 0.5 cell/well. Is there any formula to use? I think I...

21 December 2013 3,692 11 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View