i tried blue white screening nd the result was only white colonies not even a single blue. wat may the reason for tis result?
I am getting average coercivity in the range of 0.099-0.144 kOe and M - H curves obtained from VSM for the applied field upto 1.5 Tesla and 2 Tesla are almost S shape. Please provide some good...
13 June 2024 372 4 View
Can anyone help me to find out the Rs = Solution resistantce and Rct= Charge transfer resisistance of the following equivalent circuit. specifically, which R should I take as Rct. I got this...
12 May 2024 4,358 2 View
Adaptive Filters are core of the ANC system. They continuously adjusts its filter coefficients to minimize the error signal, effectively generating the anti-noise signal. Common choices include...
21 April 2024 4,468 1 View
Hello folks, I recently started using orca 5.0 version to calculate Molecular electrostatic potential but after the first step it generated .densities file instead of .scfp file (which was...
13 February 2024 7,400 4 View
Hello everyone I generated the lattice of ice using genice2 software and now i want a single unit cell from that lattice and i am unable to get that. For this purpose i tried using vesta software...
23 January 2024 7,461 0 View
Hello folks, I am trying to install genice2 software.Even after satisfying the per-reqistics and installing completely when i try to run it the error i am getting is " cell: np.ndarray[3, 3] =...
26 December 2023 376 3 View
I am trying to solvate my solute in DMSO but each time when I try to solvate using the gmx solavte command it adds only 7 DMSO molecules to the box rather than completely filling the box. Can any...
20 November 2023 9,664 0 View
The error is Cannot read module file ‘yaml_output.mod’ opened at (1), because it was created by a different version of GNU Fortran compilation terminated.
03 July 2023 9,604 1 View
I have searched on Google and got two or three answers for the same. O for oxazine or due to the ortho position of methyl group. Please clarify.
10 May 2023 8,681 2 View
Please suggest any dye that can be used to stain plasma membrane in in whole tissue after fixation and permeabilization.
24 November 2022 6,450 1 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...
26 July 2024 6,606 0 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I have conducted virtual screening using Schrödinger on a database of 17,000 molecules. Unfortunately, I cannot use the system with the Schrödinger license at the moment. I am trying to find a way...
18 July 2024 2,881 4 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?
12 July 2024 9,904 1 View
How can we measure their colony diameter?
12 July 2024 7,027 0 View
Dear all, We are producing activated carbon from hazelnut shells but have an issue about the dust of the a.c. granules also known as the dust film on granules. Linear vibrating seives doesn't do...
11 July 2024 9,782 2 View