We are working on insect cell culture using Kimura's modified media. We have observed that cell are live for near about 20 days but no growth was obs?

We have tried different pH and substrates but still no cell division was observed.

21 July 2019 2,817 2 View

Relative gene expression analysis by 2deltaCT method?

Hello, In my experiments I divided mice into 4 groups. one group fed on control diet, second group on high fat diet (hfd), third on hfd+treatment1 and fourth on hfd+treatment2. While analysing...

27 December 2018 1,455 3 View

Research vs structural consultancy...which one should be considered for future and personal development??

Life is stuck in between consultancy and research work. Consultancy gives you practical experience along with money, however, research is limited to citations, projects etc. I know both fields are...

18 October 2018 7,373 3 View

Magnetic properties of HfO2

20 June 2017 1,834 2 View

How to deposit Graphene on HfO2 with good interface quality?

20 June 2017 1,468 2 View

How to etch HfO2 when it is deposited on graphene?

How to etch HfO2 when it is deposited on graphene?

26 April 2017 549 2 View

How should I simulate accelerometer in comsol?

I am simulating a mems accelerometer L type spring . during eigenfreq. measurement displacement found is in order of 10^6 and it was supposed to be in micron. so is there any mistake that I am...

15 March 2017 1,757 6 View

Citing Product Information

I am writing a Ph.D thesis and obtained information about how a product was prepared (a purified protein sample) from the vendor. The information was exchanged via email. When I asked them for a...

07 March 2017 5,705 3 View

How to test for Ca(2+) free versus saturated Calmodulin?

Hi,I was wondering if anyone would be able to recommend a simple way to test whether a any calcium binding has occurred with calmodulin (a purified protein sample purchased from calbiochem) in a...

04 January 2017 2,379 8 View

Can anyone share their knowledge about voice analysis software?

Not the freewares like PRAAT. For sleep deprivation studies.

19 June 2014 9,724 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

How can I import .sav file into LISREL?

I've just downloaded free trial version of LISREL 10.3 but I got stuck with importing .sav (IBM SPSS 25.) file. I mainly followed the steps of this video...

15 February 2021 752 2 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to screen phytochemicals directly from the crude plant latex?

Number of methods are available to screen phytochemicals through extract prepared in different solvents including aqueous and organic. Is there any method/ published work related to phytochemical...

01 February 2021 7,348 4 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

How would you assess protein binding adjusted EC50 for a drug that has a binding rate of over 95%?

I'm in need of a reference or a method to come up with a protocol that allows one to get the protein-adjusted EC50 for an antiviral drug in presence of human serum albumin and acid glycoprotein.

27 January 2021 1,402 1 View