I run a IF staining on cultured cells. In one case, I did not apply the primary antibody to test it. It gave me signal. Why?

More Hana Marz's questions See All

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Can I transfer the 3D file from Matlab to Ansys or Abaqus?

I have a 3D output in the figure section of Matlab, does anyone know a way to transfer it to Abaqus or Ansys? Or in what format should I save it so that these analysis software can analyze it?

09 July 2024 9,226 3 View

DNA extraction from clinical sample of Staphylococcus aureus?

Salam everyone, So I am trying to extract DNA from isolates of clinical samples of staphylococcus aureus, I am using a QIAamp UCP kit. I followed the same manufacturer protocol and used Qubit to...

03 July 2024 3,892 1 View

Plant extract sterilization???

Dear researchers, I've been working on aqueous plant extract sample, then I autoclaved them in 10 min to sterilize and added some preservatives. However, some of them showed bacterial and fungal...

11 June 2024 1,040 2 View

Editora Artemis, Editorial Académica Española: Predatory publishers?

Hello, On the basis of the conferences I have attended, I was contacted first by Editorial Académica Española to publish a book with them (a development of a conference paper), so I would like...

09 April 2024 1,751 3 View

How to eliminate a recurring mold contamination in the CO2 incubators?

Dear researchers, We are struggling with neverending mold contamination in all of our CO2 incubators. In the past, we used to do H2O2 decontamination/24hr-UV mode every two months now we do it...

04 January 2024 3,717 4 View

How can we improve the sonication process to achieve homogenous fibrils of the desired length (<= 50 nm) as indicated by Dynamic Light Scattering?

We conducted Dynamic Light Scattering (DLS) before and after sonication of our preformed fibrils of alpha-synuclein (aSYN) using a microtip sonicator. Our protocol was as follows: Amplitude:...

10 December 2023 2,768 1 View

How to statistically analyze species diversity more than two communities?

Dear coluegues, I want to analyze, parkland woody species diversity along agro ecology (low-land, midland & highland). how can I check if there is a significant difference along agro ecology...

06 October 2023 9,369 1 View

What are the problems of separating imprisonment and what are the solutions?

21 September 2023 299 0 View

Can I create a new sort of Research Item?

Hi, I would like to create a new sort of Research Item (namely workshops, some with video links). Is that possible? Or it should be better included in "Presentation"? Thank you, Hana

21 September 2023 8,926 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,615 0 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,952 0 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 640 2 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,708 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,781 2 View

Richard Robohm

Some cells may have autofluorescence at particular wavelengths. The only way to avoid this is to use a different fluorescent-tagged antibody that has a wavelength that is different from the wavelength that is giving you a problem.

Nilanjana Prasad

This is a case of false postive. The 2° Ab might have bound to an Ag present in sample or any other complemntry thing