I run a IF staining on cultured cells. In one case, I did not apply the primary antibody to test it. It gave me signal. Why?

More Hana Marz's questions See All

Can I use a categorical covariate in a Generalized Linear Model in SPSS v.26?

Hello everyone, hope you are well. I am planning to use a Generalized Linear Model in SPSS. I would like to add gender to the model as a covariate, however from what I have read, only continuous...

14 February 2021 8,937 4 View

One-Way or Two-Way ANOVA?

Dear, In the preparations for publishing a manuscript, we have a big argument about the proper analysis of variance to compare multiple groups for a certain staining quantification. We have two...

27 December 2020 7,916 3 View

CD38 KO in MM cell lines?

Hi! I have been looking for publications where CD38 knock-out (or even knock-down) in multiple myeloma cell lines has been generated, but I have difficulty finding them. Have you come across...

29 October 2020 6,184 1 View

Is there a substitute to InhibitEX buffer in Qiagen Stool DNA extraction Kit?

I have a DNA extraction kit and would like to know if there is a way to make my own InhibitEX buffer (or something similar with the same function) to remove PCR inhibitors commonly present in...

23 August 2020 3,079 2 View

I need to perform antioxidant testings on Histocyctic lymphoma cells, any suggestons of another cell line, if it doesn't become available?

02 August 2020 4,502 2 View

As an academic researcher in Arab universities, what is your impression about the experience of distance education in light of the Corona 2020 ????

04 May 2020 3,099 3 View

Can PLAXIS 3D use to evaluate the liquefaction analysis?

Dear researchers, I would like to know the ability of PLAXIS 3D, which can use to evaluate the liquefaction analysis or not? If can, could you please provide me links of its relating tutorials...

25 July 2019 7,058 3 View

DNA extraction from dry sealed tissue?

Hi, my colleague stored his samples of Drosophila pupae in PCR plate. He just put samples in wells, sealed it with PCR foil and left in room temperature for couple of months. He did not add any...

14 April 2019 3,282 2 View

What can be the potential applications of alloy powders?

I want to complete my research work by studying properties and applications of alloy powders.

17 April 2018 5,529 5 View

Is it possible to buy normal plasma cells from healthy donors or RNA at least?

Hi, I need normal plasma cells or RNA as control for myeloma plasma cells. A years ago it was possible to buy them from AllCells but I could not find it. Thanks a lot. Hana

14 February 2018 3,282 4 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

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03 March 2021 6,299 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

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02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

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02 March 2021 7,829 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

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02 March 2021 9,945 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

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02 March 2021 1,146 2 View

Help request in CST microwave?

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02 March 2021 2,246 2 View

Richard Robohm

Some cells may have autofluorescence at particular wavelengths. The only way to avoid this is to use a different fluorescent-tagged antibody that has a wavelength that is different from the wavelength that is giving you a problem.

Nilanjana Prasad

This is a case of false postive. The 2° Ab might have bound to an Ag present in sample or any other complemntry thing