I tried several routs suggested in literature to perform coupling reaction between acid and amine at the Au surface using EDC-NHS in water but was not successful. Could you please suggest any better way to do this?
Sevan
You have aniline in your draw, it is not very reactive toward NHS ester. You need Benzyl amine(C6H5CH2NH2) or any other -CH2NH2 to make the reaction go forward.
And it is amide bond, not exactly peptide bond unless you use amino acid for your amine!
Thank you Guobing Xiang and Sandeep Chandrashekharappa for your answers.
I think you can use DCC for better result 📷
For other approachable method you can go to those link where you can find various method of successful bioconjugation
https://www.youtube.com/watch?v=SJEulFA2m5c&list=PLTCBtzqkKa4QhazLdinhoIuIx-HV6YDCX
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