I need to analyse the total nitrogen, nitrate nitrogen , ammonia, ortho phosphate and total phosphorous in the paddy plant at different stages?

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Kamal Hama Karim

This link may help you.

https://lter.limnology.wisc.edu/protocol/total-phosphorus-and-total-nitrogen

Anoop Kumar Srivastava

Very informative link..worth reading Kamal

Paul Milham

No. A dilute strong acid extraction will give phosphate and nitrate, and possibly ammonia, but you would need ref samples to validate this. Total N and P require stronger treatment, e.g. they can both be measured on a Kjeldahl digest, but do not use Se as a catalyst if you want to use the Murphy and Riley method for P