Cannot you use rating scales of other pathogenic Pseudomonas?? If not rating scale for  Pseudomonas tolaasii avilable?

yes,i can use but better is that....if possible

Dear , Muhammad Idrees.

You can see the following attached paper .

With my best regards

Thanks alot sir @ yehya A Salih...

Dear Muhammad,

You can see the following paper:

Geels, F. P. (1995). Pseudomonas tolaasii control by kasugamycin in cultivated mushrooms (Agaricus bisporus). Journal of Applied Bacteriology 79 (1): 38-42.

Best regards,

Francisco J. Gea

Thanks Francisco J.Gea

You can use fresh cubes (1-day-old A. bisporus) excised from the caps with around 2 cm apart (to avoid cross contamination) . Test bacterial suspension  (10 8 CFU ml-1 of your isolate of P. tolaasii could be simply spotted over the surface of  cubes (No.   ?) in comparison to control. Check daily your inoculated cubes incubated at 22°C for color change (mainly tissue' browning scale). Kindly have a look on my published paper in 2012 (www.ncbi.nlm.nih.gov/pubmed/24031938).

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Hi Muhammad

You can see the scale in this paper "Biological control of mushroom brown blotch disease using antagonistic bacteria"

http://www.tandfonline.com/doi/abs/10.1080/09583157.2013.873113?src=recsys&journalCode=cbst20

thanks sir...@ Mohamed A. Abouzeid