What is your suggested method and materials for producing UV-curable transparent coatings?

What are the easy methods for synthesizing UV curable resins? I need to synthesis UV curable coatings. MMA seems to have good features for transparent coatings, but the additives and synthesizing...

24 April 2020 3,256 7 View

What is the command for running comsol in the linux os?

I'm using comsol multiphysics on a cluster that operate linux os. i used this command to run and solve my mph file that named s4: comsol batch inputfile s4.mph outputfile s4solve.mph but the...

25 February 2020 5,691 1 View

How can one learn about the effects of an organic substance on the human body?

For example, we synthesized organic matter called A, at this point we want to know its effects on the human body. I wanted to know if there was a special device for this or were they using other...

16 December 2019 2,223 3 View

In immunofluorescence assay, do we need to wash away (with 1X PBS) blocking buffer (10% FBS-PBS) before adding the first antibody?

In immunofluorescence assay, do we need to wash away (with 1X PBS) blocking buffer (10% FBS-PBS) before adding the first antibody?

25 September 2019 6,591 3 View

How can I attach an amine group (-NH2) to a carboxyl group (-COOH)?

20 September 2019 5,256 5 View

Is it possible to simulate hysteresis effect for piezoelectric material in COMSOL or ansys?

I tried sinusoidal input voltage with the amplitude of 100 V but couldn't get any hysteresis curve

24 May 2019 5,973 4 View

Taqman probe real time PCR...Problem regarding constructing plasmid dna standard curve?

Hi guys......i am facing problems regarding developing standard curves for different copies of plasmid DNA using taqman probe real time PCR. i have successfully obtained standard curve for...

06 March 2018 2,284 3 View

Plasmid DNA copy number dilution?

Hello everybody..... the concentration of my plasmid DNA is 126ng. i want to do real-time PCR taqman probe detection......i want to do serial dilution that should start from 10e8 copy number....so...

21 February 2018 3,230 3 View

Looking for a learning tutorial for TSOL software?

Hello I am trying to find a learning tutorial for TSOL software. I would be thankful if you help me in this way Best Regards Mehran Dehghan

27 October 2017 8,799 3 View

Is there any isoform of Rattus norvegicus TGF beta1 mRNA?

I want to do SYBR real time PCR for relative quantification of TGFB1. Gel electrophoresis of real time PCR product showed two bands in around about 80 bp (79 oC melting peak) and 180 bp (84 oC...

22 July 2017 2,265 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View