I want to evaluate the humoral immune response to adenovirus vector vaccine carrying the HA gene of A/HongKong/156/1997/H5N1. I am working in BSL2+ lab, But I don't have this virus in my stock. I evaluated the cell mediated immune response but, I want also to evaluate the humoral immunity.
Do you have any suggestions?
Protein Sciences sells rHA from several viruses, including HK/156/97. http://www.proteinsciences.com/
hello Thomas,
I checked with the company you mentioned, but they told me this protein is not in the regular inventory. It needs to be made to order and it is so expensive. Do you know other ways to get this protein?
Thanks,
Ekramy.
Unfortunately, this virus is listed as a Select Agent and would require special clearances from Select Agent Program. Another source of the recombinant antigen could be the International Reagent Resource program (www.internationalreagentresource.org/) which we run through CDC. You can check with it and see if it is available or you need to setup a collaboration with us in order to get it. Give it a try.
Thomas
Thanks Thomas;
I checked IRR. They Don't have it.
But, my lab has already a collaboration with the CDC. I am working in Dr. Mittal's lab.
But I don't know how to ask them for the reassortant virus?
and I don't know if they have the rHA if I can not get the virus.
Can you tell me how can I ask for it?
Thanks,
Ekramy.
You can contact Dr. Todd Davis ([email protected]) who may be able to help you. Good luck.
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