Is there some specific drug to induce injury only in muscle fibers without affecting peripheral nerves?

I would like to induce injury in skeletal muscle fibers to study muscle regeneration but I would like to preserve nerve terminals intact. Could it be possible? Is there some specific drug? Thank...

30 October 2020 9,077 4 View

How do I extract data Hazard Ratio and CI from Kaplan-Meier curves?

I am conducting a meta-analysis of Hazard Ratios (HR), however, most of the studies presented only Kaplan Meier curves without the HR and Confidence Intervals (CI). I am extracting data manually...

23 June 2020 6,584 1 View

How can I solubilize HgNO3 in water?

I need to solubilize HgNO3 in water at different concentrations, but I have notice an orange precipitate formation when I dissolved the salt in water. Probably it is mercury oxide. How can I...

28 March 2019 1,277 3 View

Procedure for thawing cryopreserved hpc sample for flow cytometry viability via 7-aad?

Currently our lab performs post thaw viability via trypan blue. We would like to begin using flow cytometry 7-aad for our post thaw viability. Our attempts so far at thawing our samples have led...

22 September 2018 5,396 3 View

Analysing questionnaire with multiple constructs in SPSS

27 May 2017 9,677 5 View

How to account for phylogenetics in variables (phenols, nitrogen, leaf toughness etc) for australian plant families? PGLS? where do you find the data?

I have a data set of chemical and physical traits (and herbivory data) from trees and vines at one of my study sites. Unfortunately, there was no overlap in the families trees vs vines came from...

26 February 2017 3,263 3 View

It is possible to detect (by WB) a membrane receptor, mainly in the cytosolic fraction after a differential centrifugation?

07 December 2015 5,342 3 View

Aside from looking to prototypicality studies for category lists, are there more sophisticated and open-source databases available (i.e. animals)?

I am looking to generate culturally unbiased and medium frequency words to be used in a memory task.

23 March 2015 4,056 3 View

Has anyone detected BDNF in tissue homogenates by ELISA?

08 January 2015 3,081 7 View

What is the effect of Arginine on siRNA binding?

23 July 2014 363 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View