I m looking for human sensory neurons as primary cells, can anyone please suggest me where i can buy them?

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COMSOL simulation on TENG?

Hello. I am trying to do contact-separation mode TENG. I have been following , and I am suffering to calculate the Open circuit voltage (Voc). I made geometry and set the materials setting all...

26 May 2024 2,645 0 View

How to validate agonist and antgonist of CB1 receptor (check CB1 activity) in tissue?

We want to block and potentiate CB1 receptor in tissue through agonists and antagonist "ex vivo". But how we can check that the drug used is working? We can check by WB the levels of the total...

22 April 2024 8,060 0 View

What is the holding time for a digested water sample for total phosphorus analysis?

I have carried out an acid persulfate digest (sulfuric acid + potassium persulfate) for total phosphorus extraction on freshwater samples, but can't find a reference for recommended holding time...

10 September 2023 1,438 3 View

What is the difference between these two data in triboelectricity open circuit voltage?

I am studying about triboelectricity, and I have a question why some open circuit voltages some out as single-peak and some output voltages comes out as double-peak. 1. The first one has only one...

03 September 2023 5,044 1 View

What are the consequences of not participating in EQA for Neisseria gonorrhoeae antimicrobial susceptibility testing?

How might the decision of laboratories or healthcare facilities to skip participation in an External Quality Assessment (EQA) program impact the accuracy and reliability of Neisseria gonorrhoeae...

02 September 2023 6,303 2 View

Do people often purchase bird nest boxes for research (pre-assembled, kits to be assembled) or mostly build boxes themselves with raw materials?

Do kit nest boxes hold up well in the field? What brands have you had success with? Is the cost trade off between do-it-yourself vs buy a kit worth it?

12 June 2023 720 0 View

Is this a good model for Repeated Measures Mancova?

I surveyed 400 participants and each participant received 8 images to analyze, each image with the manipulation of 3 factors (A, B, C, AB, AC, BC, ABC, Control - no changes). Then, they answered...

22 April 2023 10,016 3 View

Can I use dry roots for Root Scanning WinRhizo to get data ?

I am wondering if I can use dry roots to get data generated by WinRhizo when I scan roots? I have different treatments and want to scan dry roots to get data from WinRhizo. Most papers I have...

16 April 2023 1,957 0 View

How to create dummy variable for within subjects?

I am running a mancova where each participant received 8 versions of a phishing email that combines 3 factors (A,B,C,AB,AC, BC, ABC, Control). I am measuring the level of risk (4 levels). My...

05 April 2023 8,425 1 View

How to do a Pairwise comparison for interaction effect using Repeated Measures Mancova in SPSS?

Hello everyone, I am using a Repeated Measures Mancova with 4 factors, each one with 8 groups. I also included 3 covariates in the analysis. I discovered there was a significant difference...

25 March 2023 3,216 5 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

An animal in not a rock (e.g., Koch 2013), it has an internal constitution that can be modified?

Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...

07 August 2024 648 0 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Are the GABAergic neurons electrophysiologically equal in all brain areas?

Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?

04 August 2024 4,090 0 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View