I have to prepare nanoparticles of a freely soluble drug, can I keep external phase as water? I suspect that drug may not dissolve in aqueous phase?

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Staining of foam cells derived from PBMC differentiated to macrophages. Does anyone have experience with this type of assay?

Hi everyone, I am trying to induce foam cells in macrophage differentiated from PBMC. Which type of staining do you prefer to use? Nile Red or Oil RED O ? What concentration and timing of ox-LDL...

01 July 2024 8,286 0 View

Which negative control do you recommend for In vitro Foam Cell Formation Assay?

Hi everyone I have to perform the Foam Cell Assay with OIL RED O staining. Can you recommend a compound that inhibits LDL oxidation? Also, can anyone recommend oxLDL to use?

14 May 2024 6,404 1 View

Can I transfect differentiated cells?

In particular using a DNA plasmid transient transfection on differentiated skeletal muscle cells?

29 April 2024 9,064 2 View

Research article about minors community?

i need some information to build a thesis about minors community

22 April 2024 1,081 0 View

What do you think I'm doing wrong?

Hi everyone, I differentiate THP-1 in macrophages with PMA 48h + 24h rest. After treatment with LPS I would expect an increase in ROS. But this increase compared to control I cannot get. I have...

25 March 2024 4,797 6 View

Which statistical test should I use?

Hi, I need some information. After collecting data from various experiments, I am proceeding with a statistical analysis. Since I work with frequencies, I was told to use the chi-square test....

20 March 2024 5,496 4 View

When does the Griess colorimetric assay produce yellow instead of pink?

I am using the Nitrate/Nitrite Colorimetric Assay Kit from Cayman. When I used the nitrate+nitrite determination assay with nitrate reductase on the same samples' supernatant, it turned pink. When...

18 February 2024 3,338 0 View

Hello there, any suggestion to calculate equilibrium constants without NMR variable T?

If we have an host-guest complex, in water an equilibrium occurs. So you can have ComplexGuest + H2O ComplexFree + GuestFree. So I can calculate equilibrium constant, but in the most of...

31 January 2024 335 1 View

What medium can I use (instead of RPMI) on differentiated THP1 if my goal is to measure small changes in nitrate levels?

I differentiated THP1 cells into macrophages. Subsequently, macrophages will be treated with substances. Next, the Griess Reagent test will be used to determine nitrate levels. The Griess kit...

24 January 2024 9,551 1 View

Why are my articles linked to another account?

I've registered on ResearchGate today but I can't upload my articles because they are linked to another account with the same name: Giulia Errico, Zurich University of Applied Sciences,...

29 October 2023 1,270 1 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

I want to buy Hydrothermal Synthesis Autoclave from any European company. Can anyone suggest any company inside Europe?

Thanks

22 July 2024 1,143 3 View

Hi dear researchers, do you suggest the best and shortest path for chemical synthesis and green synthesis of magnetite(Fe3O4)?

Suggest the best and shortest route for the chemical synthesis and and green synthesis of magnetite(Fe3O4) The chemical synthesis should contain the least dangerous chemicals along with full...

21 July 2024 2,476 2 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

19 July 2024 8,522 0 View

How to extract binding energy from pv.maegz file without using Schrodinger?

I have conducted virtual screening using Schrödinger on a database of 17,000 molecules. Unfortunately, I cannot use the system with the Schrödinger license at the moment. I am trying to find a way...

18 July 2024 2,881 4 View

Use of NC or NTC to descibe negative control?

Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...

18 July 2024 4,183 2 View

Im trying iodination of an arene, but only getting black precipitates, is there anyone with iodination experience who can advise?

Organic Synthesis. My student has been trying iodination of arenes (our goal is to iodinate p-phenylenediamine) following these literature procedures: 1. Synthesis 2004, No. 11, 1869-1873 using...

16 July 2024 6,630 1 View

What could be the possible reason that sintering of polycrystalline diamond fabricated with tape casting has suddenly started failing?

I make polycrystalline diamond with tape casting followed by HPHT sintering. When i sintered the samples up till May, the sintering was good. When i sintered the samples in June and July at the...

16 July 2024 3,212 5 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

15 July 2024 2,414 1 View