Is GAPDH a suitable reference gene for qPCR in transfected cells?

I transfected Huh 7.5 cell lines with vector pDG461which harbors Cas9. I performed qPCR to make sure that Cas9 is expressed in my cell lines. I used GAPDH as a reference gene in qPCR and my...

11 July 2020 7,139 11 View

Would you please introduce me a functional and updated protocol's sources for biotechnology research ?

Hi everyone I am looking for a precise and comprehensive source for protocols of laboratory techniques which are used in biotechnology or cell culture and related subjects .Do you know guys any...

21 May 2020 889 8 View

Why is a DNA partial sequence (16s rRNA) placed in a separate branch in phylogenetic tree?

Dear all, I have some bacteria DNA partial sequences (16s rRNA). They sequenced only by 9 F primer. Their length is under 1100 bp. When I draw phylogenetic trees for some of them by MEGA, my...

23 April 2020 9,203 8 View

If there are any questions, colleagues need me assit ?

Hi Dear colleagues Since I am not always sitting behind computer to respond your questions. Please feel comfortable to contact my what app no:+994505618799 ,if there are any questions I could be...

31 January 2020 2,922 3 View

What is the effect of surrounding medium, e.g; Acidic nature, on spectral variation of LSPR for the Au nanoparticles?

I want to know origin of spectral shift in my plasmonic sensor when medium becomes more and more acidic.

08 October 2019 4,247 3 View

What is the best protocol for operating protein SDS -PAGE and Western blot ? Is there any video to learn more about details and trouble shooting?

hello every one I am looking for a really precise protocol for doing protein SDS PAGE and also Western blot and of course a good source for how to deal with their trouble shooting . any kind of...

23 August 2019 2,104 5 View

How is stabilizing NeoRez R981 Polymer with Triethylamine?

I was reading the patent " Method of providing a substrate with a hydrophilic coating and substrates, particularly medical devices, provided with such coatings", and in one of the examples, it...

21 December 2018 4,397 3 View

What is the best source for learning Comparative Genomics ?

Hi I am looking for a really functional source for learning more about details on the topic of Comparative Genomics .I would be appreciated to introduce me couple of them .

16 November 2018 2,647 9 View

How to calculate concentration of drugs in small intestine?

Does anyone know if there is any formula I can use for calculation of a drug concentration (knowing oral dose) in small intestine and/or other parts of GI? Thanks

20 February 2018 2,352 1 View

What are the most important characteristics affecting the suitability of an area for flood spreading purposes?

Dear Experts, Based on the literature review, slope, land use, infiltration rate, alluvium thickness, and EC are some important factors. Is there any other important factor in these kind of...

23 November 2015 3,765 15 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View