I have RNA-seq files that are in Fastq format but are in GZIP type. I want to convert or open this GZIP files into Fastq. What is the best software to use for this purpose?
On UNIX: gunzip FILENAME
On Windows or Mac OSX, any compression software (WinRAR, 7zip etc.) should be able to unzip them. (Just Google!)
Note that gzip is just *compression*, it is not a change of format as such. You just need something to uncompress the files. Note also that text (such as fastq) can be compressed *a lot* and so the uncompressed files might be very big - depending on your disk space, you might need to gunzip, process and then recompress (e.g. gzip) before going on to the next one.
Thank you so much Richard, I will try and will inform you it this works.
>gunzip file.fastq.gz should work.
What will you do with your fastq files? Have you considered using an online NGS data management resource like InSilico DB https://insilicodb.org?
Results example: https://insilicodb.org/differential-gene-expression-heatmap-from-rnaseq-data-using-cummerbund/
Hi Dear Vivian Panes For your answer now it is so late!!!! but if other researchers now the same problem, you have asked an applicable question.
I use the bellow link in windows OS system that is a good and useful program. You can apply it easily for solving your problem. Just try it! https://tool-download.com/System-Utilities/File-Compression/PeaZip Regards, Nasrin
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