Matrigel is at the lower and upper of cells. I want to isolate cells from the plate and matrigel and use them for WB. I used Cardiomyocyte Dissociation Kit Catalog # 05025 but It solved the matrigel.
Dear Nazanin!
Maybe this protocol will suit you, please see the attached file
https://www.miltenyibiotec.com/DE-en/applications/all-protocols/differentiation-isolation-and-analysis-of-cardiomyocytes-derived-from-pluripotent-stem-cells.html#gref
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