09 February 2021 4 1K Report

Till now i haven't got any PCR amplified vp2 gene bands on specific annealing temperature and in step up PCR.

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How to quantify the fluorescence intensity of only the merge area of an image using ImageJ software?

Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...

22 July 2024 8,770 1 View

How to draw a graphs of bioconcentration of pesticide and its relationship with log Kow, log Sw and molecular weight?

Help me to draw graph of Bioconcentration

10 June 2024 4,941 2 View

How to normalize fluorescence measurements from different microplate runs?

I'm currently doing an experiment where I'm following the expression of a reporter gene (GFP) along growth using S. cerevisiae. The cultivations last around 48 hours. I observed that the results...

10 June 2024 5,941 3 View

I want determine Iron by spectrophotometer? Anyone know about the spectrophotometric determination of iron and copper, kindly share the procedure?

spectrophotometric determination of iron and copper

04 June 2024 1,720 0 View

How to estimate total sugars using lane and eynon method?

I want to estimate total sugars in carrot and products developed from it. I have been using lane and eynon method. I could get the values for reducing sugars but not for total sugars. What is the...

28 May 2024 9,382 4 View

Dissertation topic for MSC Computer Engineering Students?

My name is kiranbir kaur and m student of MSC Computer Engineering in Solent University Southhampton (United Kingdom).Please suggest me the topic for dissertation.Include the Problem and Solution.

24 May 2024 4,355 2 View

Dissertation Topic for MSC computer Engineering?

Hi, My name is kiranbir kaur and m student of MSC Computer Engineering in Solent University Southhampton (United Kingdom).Please suggest me the topic for dissertation.Include the Problem and Solution.

24 May 2024 1,180 3 View

Why total dissolved solids (TDS) are not reduced after treating waste water by vermifiltration process?

Dear researchers, I am treating the waste water by vermifiltration process using different filter bed media. After the treatment, BOD, COD, TSS were found to be reduced but reduction in total...

18 May 2024 1,897 3 View

How to eliminate the periodic functions in Eq.3?

The equation in fig.1 can be transformed to the one in fig.2. Using ‘atan’, we can turn the periodic function ‘tan’,which is on the left of Eq.1, to the sum of ‘atan’ and l*π. However, for the...

14 May 2024 8,585 4 View

What formula we use for calculation of anthocyanin from optical density values?

Using 10 ml of 1% methanolic hcl, 1 g fresh sample and OD at 530 and 657nm

07 May 2024 4,440 1 View

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