I have a peptide sequence of length 51 amino acids for which the gene sequence is unknown. How do I amplify the concerned gene?

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Alireza Mordadi

Did you tblastn(protein to translated nucleotide)?

Michiel Vanmeert

Hi,

If you know what species your gene is coming from, you can convert the amino acid sequence to nucleotide using for example the backtranseq tool from EMBL. This of course of your tblastn was not successful.

Probably the entire genome of the species that you retracted the peptide from is not known ?

Shashidharan Anisha

Thanks for the solution. I'll try and come back.