I have found a circulating antigen in plasma of cancer patients also its autoantibody by indirect elsa? Is it possible?

i have used indirect elisa

23 October 2016 9,695 1 View

Can I perform phenol-chloroform-isoamyl alcohol (ratio 25:24:1) dna extraction from bacteria without using CTAB? If yes, then how?

I am currently working on extracting dna from H. pylori culture isolates by phenol-chloroform-isoamyl alcohol method by using CTAB/NaCl. Can I use the same method of extraction but without using...

28 June 2016 1,582 5 View

I have a solution containing insect digestive proteins and bacterial proteins.How can I separate them?

Are there any protein kits available to do this?

21 April 2016 8,774 3 View

How to make primary leukemic cells take up the annexin stain when undergoing apoptosis?

Some leukemic cells collected from patients undergo apoptosis when treated with a drug. Immunoblotting shows complete PARP cleavage.On the other hand, when I did Annexin V/ PI staining on the same...

14 August 2013 8,319 4 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View