I have a method in my thesis wherein i should dilute my 1 x 10^8 CFU/ml to Make it 1x10^5 CFU/ml. Is there any way this is possible?

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How to interpret MIC values in microplate reader?

Good day! I am currently testing my sample for MIC, may you help me how to interpret the results at OD630? how would I know if there is growth or not based on the values given by the microplate...

01 June 2024 7,920 3 View

Why do you translate my name?

My name is Mary Elizabeth C. Bernardes -de-Oliveira or M.E.C. Bernardes-de-Oliveira. But, Maria there isn't! Please correct this for me.

27 May 2024 8,228 2 View

Apart from basic bone remodeling markers, which other blood biomarkers do you think have potential to predict fractures in older adults?

Currently, there are numerous blood biomarkers that have been linked to fractures and increased fracture risk (for example, IL-6). Considering the current evidence, which ones do you think are the...

10 May 2024 6,097 2 View

What are your free yet efficient go-tos tools for meta-analysis?

Need advice on user-friendly tools to ease meta-analysis process of selecting eligible and fugitive studies

02 May 2024 2,332 3 View

What cut off values should I use for my phylogenetic tree?

Hi, I am currently constructing a phylogenetic tree involving heterokonts, specifically of marine diatoms and dinoflagellates. Looking at the BLASTn results of my query sequence, I am wondering...

30 April 2024 4,239 2 View

Why do i have 2 bands in γ-tubulin in tissue?

The γ-tubulin Ab i got gives me a 48kDa band in hepa cell samples but when i try it in mouse liver tissue samples i get a weird band at 48 and another one at almost 70kDa. Ive tried a different Ab...

23 April 2024 9,475 0 View

How to respond to request for copy of article?

Someone has requested a copy of an article I co-authored but I don't see options to respond. When I clicked on "View Request," it took me to a page with information about the article but not...

19 April 2024 6,368 1 View

Why is functionally curing the HBV initially preferable? Does this contribute to a higher serological test's accuracy in identifying HDV antibodies?

With accordance to the systematic review and meta-analysis conducted by Zhenzhen Pan et al., about the Diagnostic Efficacy of Serological Antibody Detection Tests for Hepatitis Delta Virus. This...

29 March 2024 5,284 0 View

Can the XRD intensity peak of magnesium doped ZnO be more than the undoped ZnO?

Can the reasons for such an incident be detailed?

13 February 2024 2,795 4 View

What is AI? And how does AI relate to the Law?

Kindly research on how AI is going to impact on legal sevices and in particular arbitraration.Right now some firms have started using AI for research,due diligence and data analytics.

12 February 2024 4,005 5 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What exactly is it about biotechnology and medical research?

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

28 July 2024 3,798 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View