Is the equipment nuclease free so as not to degrade your sample? What volume are you loading? Typically need around 200 ng to visualize on agarose. You should get a 18s and 28s band. If sample is degraded you will only see a smear
To improve visualization of RNA bands on the agarose gel when no bands are observed despite having 150 ng/μL of RNA, you can:
1. Check gel concentration.
2. Optimize gel electrophoresis conditions.
3. Try a different gel stain.
4. Include RNA size markers.
5. Verify RNA integrity.
6. Consider alternative visualization methods if needed.
The concentration seems too low. I think you need to re-extract the RNA from your sample and recheck the purity and concentration. You can run it on agarose gel if the concentration is more than 200 ng.
The other factors affecting RNA quality are high voltage and contamination as RNA is highly unstable. Be cautious while the RNA extraction process. I hope this will work.
The RNA concentration is too low to be visualized on an agarose gel.
Increase the concentration of RNA and make the gel running condition nuclease-free. Hope it will work.
RNA concentration is low. Also, storage of RNA matters and it degrades easily. Always store right away in -80 and make 5-10ul aliquot after extraction for gel visualization and quantification. if it degrades, you can use the stock.
https://www.thermofisher.com/tr/en/home/references/protocols/nucleic-acid-purification-and-analysis/rna-protocol/agarose-gel-electrophoresis-of-rna.html
You have to increase concentration of RAN and check concentration of agarose gel.
Did you get the value of the concentration by Nanodrop? Remember that Nanosrop gives a value but does not discriminate between degraded material or not. What is the detection method? What is the RNA extraction method. I consider that the initial concentration is low. I suggest placing another known sample on the same gel and testing.
You Need to enhance the concentration of RNA, Gel electrophoresis condition, post staining procedures.
you must use RNA immediately after Extraction ,I suggest placing another fresh sample on the same gel and testing.
Firstly check RNA integrity, if it's fine then you should increase conc. of RNA.
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