Dear Chanaka Sandaruwan

Please, not try to worm the solution to avoid the destruction of many sensitive phytochemicals. The best choice is to use aqueous ethanol even in large quantity.

All the best

අම්ලයක් දාලා බැලුවේ නැද්ද දියවෙනවද කියලා

No.

We are planning to introduce some dispersion agents to enhance the solubility.

Did you try warming your solution? The target concentration is another issue and so, first try to make a low concentration of this powder.

Hi,

I agree with Stjepan Krešimir Kračun comments; low solubility / hydrophobic final extract ingredients are expected and oxidation possibly undergoes at your product.

With respect to the solubility and stability issues, I have several suggestions that could be tested.

Try to use hydrotrope agents and their solutions (such as benzoate, salicylate, Sodium cumene sulfonate, Tosylate, Hydroxylamine hydrochloride, Meglumine)

Chaotropic agents like urea or basic amino acids could also improve solubility but these agents make the product more prone to be oxidized. Antioxidant supplements (such as citrate, ascorbate, tocopherol, etc.) should be added at excess quantities but also you need to be sure for minimal air exposure for inhibition of the redox reaction.

As a third and novel option, deep eutectic solvents (DES) or NADES may work, if the concentration of the active substance at your final extract is not very high. These solvents present satisfactory solubility and extraction options for hydrophobic substances at aqueous media. Diverse mixture and applications are available, choline based DES are typical ones.

Good luck,

Emir...

Zahir Hussain Thank you for your comment. I'll make low concentrations of this powder and check the solubility.

Stjepan Krešimir Kračun

I tried with a water/ethanol combination also. But couldn't see much difference. Also, it seems, as u mentioned this can lead to oxidization.

İsmail Emir Akyildiz Thank you for your comment. I can try the dispersion agents that you have mentioned. Especially benzoate etc.

However, I may need to work with LD50 values since the final product will be a consumer product.

Dear Chanaka Sandaruwan

Please, not try to worm the solution to avoid the destruction of many sensitive phytochemicals. The best choice is to use aqueous ethanol even in large quantity.

All the best

The questions is what you are trying to extract from this powder ? What about reversing what you have done already ? Just add some ethanol solution.

My commnets (perhaps will taste bitter to you):

1. Why you are doing such experiment? Generally such experiments are performed to validate the traditional claims of health benefits of a medicinal plant part.

Is there any report of use of any solvent extracted part of the plant as any medicine by anybody?

The answer is definitely NOOOOOOO!

Then why are you doing that? Why are you not testing the actual usuable form of the plant as reported (generally succulent form)?

It is ue to the fact that all other contemporary researchers doing the same , is not it?

I have also performed such thing in my student life.

All of us are doing the same mistake!!!!!

2. The solvent extracted portion of any dry plant part powder contains ONLY the phytochemicals able to be diluted in that particular solvent. Many of these may not be diluted in another solvent.

In your case, the first solvent is Ethyl alcohol and the second is water.

You will also should not expect similar results in the diluted plant products in both the solvents.

Please read the following conference paper abstract:

The study was designed to validate the contemporary method followed for study of anti-microbial effect of medicinal plants by methanolic extract of plant parts. Mikania scandens (L) Willd. is reported to have wound healing activity. Leaves of this plant was collected, washed thoroughly, dried under shade and extract of the leave powder was taken out by using 70% methanol and shaking by rotary shaker for twelve hours. The methanolic extract was then concentrated by vacuum evaporator and air dried inside the laminar flow. 200 milligram of the pasty plant leaf extract was mixed with 1 milliliter of 70% methanol and another 200 milligram was mixed with distilled water. Both the solutions were tested for anti-microbial effect on Staphylococcus aureus by disc diffusion method keeping Ceftriaxone as control. The methanolic extract of Mikania scandens dry leaves diluted in 70% methanol showed 23 millimeter diameter of inhibition zone against 24 millimeter showed by Ceftriaxone. The methanolic extract diluted in distilled water showed no zone of inhibition. The methanolic extracts made from the dried parts of the medicinal plants are studied worldwide to identify the anti-microbial effect after mixing with methanol. This contemporary method is questionable as the methanolic extract failed to show any anti-microbial effect itself when added with water…….

Source:

Augmentation of antibacterial efficacy against Staphylococcus aureus by methanolic leave extract of Mikania scandens (L) Willd. when diluted in methanol.

[Conference: 2nd International Conference of Indian Pharmacological Society (West Bengal Branch) (IPSWBCON – November 2013). At: Nilratan Sircar Medical College and Hospital, Kolkata, West Bengal, India].

Available at: https://www.researchgate.net/publication/261674779_Augmentation_of_antibacterial_efficacy_against_Staphylococcus_aureus_by_methanolic_leave_extract_of_Mikania_scandens_L_Willd_when_diluted_in_methanol

Thanks

By using vortex or ultrasonic.

following

Well,

Using an organic solvent, then inorganic, the extract will lose many of its active substances ... You must use an organic extract and then dissolve it with a similar solvent.

Regards

Ethanol extraction gives tannin, polyphenol, flavonol, terpinoids, sterol, polyacetylene and alkaloids etc.

these are not readily soluble in water.

if it want to dissolve in water and make solution, better option is aqueous ethanol.

if it is prefer for internal use in any condition, give as it is in specific dose.

why you are trying to dissolve in water again?