i need help
for alignment search? https://blast.ncbi.nlm.nih.gov/Blast.cgi
Commercialization of space activities and private sector investment requires the creation of a new legal system that creates the necessary incentives . Are the basic principles of space capable of...
20 May 2024 4,922 1 View
Searching for new scientific methods to reduce desertification
08 February 2024 9,280 3 View
Dear all, I have worked on the gas uptake with MOFs and now I need to calculate IAST selectivity and HOA. I've used python code which is published in some papers but it did not work for me. I...
25 December 2023 5,090 0 View
Hello ResearchGate community, I am replicating differential expression analysis from a paper (here) on RNA-Seq data. I extracted the regulated genes using DESeq2::results with a threshold of 1...
13 December 2023 6,977 1 View
Are international legal principles absolutely not reviewable , Under what conditions can the principles be revised ?
07 September 2023 3,943 5 View
What analysis, SEM or TEM, is better for MOF identification? Unfortunately, I only have one choice. I have the crystal size average with XRD, and therefore, I think SEM is better. please help me.
25 May 2023 9,390 4 View
Hello, Can I by any means open .raw files that open by XCalibur by DataAnalysis Bruker Daltonics software ? Thank you
11 November 2022 6,513 1 View
Hi colleagues. I need a simple and cost-effective method for the preparation of gamma-cyclodextrin. Please help me with this.
19 September 2022 1,197 0 View
I did oxidation of isopropanol to acetone in water and reflux conditions. Acetone is very volatile to identify with TLC and also the iodine chamber. How can I identify it?
13 August 2022 8,869 4 View
I prepared hydrogel from extracellular matrix and measured the absorbance at 405 nm during 2 hours then blotting the points in excel to calculate speed of the gelation (s) , t lag and t50%. I...
08 June 2022 2,840 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,666 3 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,101 0 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,113 3 View
As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....
05 August 2024 9,365 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,056 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,786 4 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,976 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
