CFU assay
I am searching about a software for analyzing of electronic and communication boards viewpoint of radiation tolerance. for example the output of this software is 40 krad. it means this boards can...
17 June 2019 3,414 6 View
thermoluminescence properties
09 August 2018 8,756 3 View
Details of experiment: - Concentration of TiCl4 aqueous solution=0.15 M - Temperature=70 'C - Time=30 min In my experiment, the TiCl4 solution was completely transparent at first, and at...
04 April 2018 5,726 4 View
For IP and ChIP experiments, is there any advantage in covalent coupling of the antibody to magnetic beads, over the use of protein G coupled beads? I'm thinking in terms of background noise,...
17 May 2017 2,887 1 View
I need to culture a male Drosophila cell line that is easy to culture, propagates at a great rate, and it is relatively easy to transfect. I'm also looking for a constitutive expression vector...
16 September 2016 1,605 3 View
I have about 200 data and a feature vector of more than 150 features in size. no matter what classifier or what features I use (or apply KPCA), I can't get a higher accuracy than about 70% it's...
31 March 2015 8,572 10 View
I got embryonic stem cells 1 month ago and cultured them according to the protocol from its company. After 3 X making them passage, they start growing very slowly and not confluent well as we...
30 September 2014 3,292 12 View
I tried to culture MEF feeder layer cells using MEF media which contains: DMEM containing 10% FBS, 2mM glutamine, 1x10-4M nonessential amino acids, and 50 U and 50 mg /ml penicillin and...
02 May 2013 6,394 1 View
I thaw a vial of MEF feeder layer cells of irradiated mouse embryonic fibroblast (iMEF) feeder cells from CF1 mice (GlobalStem), and culture in high glucose DMEM supplemented with 10% serum,...
12 April 2013 7,303 20 View
One of our suppliers introduced me to Cultrex® BME PathClear and Geltrex® Matrix as the alternatives to matrigel. I was wondering as if anybody has experience of using these products?
05 March 2013 6,239 2 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
