I carried out GST assay according to Habig et al and now am finding difficulty in calculations?

More Lalita Singh's questions See All

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

How to increase citation in Research Gate?

How to enhanced h-index in Research Gate?

04 August 2024 3,368 4 View

How to do wavelet transform of EXAFS data?

I want to do wavelet transform of some compounds for their comparative study? Kindly help me in this regard and provide with proper procedure.

31 July 2024 4,170 0 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

How to use energy flexibility in inventory modeling?

29 July 2024 2,192 5 View

I want to get updates regarding international law, international trade law, international institutions and human rights etc?

Please send me updates regarding the above-mentioned areas in the question mark?

28 July 2024 5,197 3 View

What is the principle/mechanism behind aging of carbon (graphite) containing refractory mix for isostatically pressed refractories?

The resin bonded carbon containing refractories are aged before use. How time of aging is determined? And mechanism behind aging.

23 July 2024 3,205 0 View

How to remove cell debris after tissue dissociation?

I'm facing issues with the tumor dissociation protocol for GBM. I use collagenase/dispase with DNase to dissociate my tumor sample. However, even after incubating it for 60mins, I do not get a...

22 July 2024 412 3 View

What methods can be used to minimize environmental variability in breeding trial ?

In breeding trials, environmental variability can significantly affect the results, making it challenging to accurately evaluate the performance of different lines and hybrids.

22 July 2024 3,961 4 View

Is profit margin a good variable to measure the market power of a company?

I have read papers where markup has been taken to measure market power. However, if markup data is unavailable, is it okay to continue with profit margin, as when a company has market power, it...

22 July 2024 707 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Why do these abnormal charge/discharge curves of coin cell batteries appear?

As shown in the figures, there are several abnormal charge/discharge curves.Each of them may have different problems. I am curious why these situations appeared differently?There are a lot of...

24 July 2024 6,930 1 View

Is this cell cycle analysis correct?

When I applied the cell cycle algorithm to these two samples in FlowJo, I encountered some concerns regarding the accuracy of the fit. The images include both a control and an apoptosis-inducing...

18 June 2024 1,984 0 View

How to prepare 0.28 U/ml working solution of Acetylcholinesterase inhibition assay for anti-Alzheimer activity?

I have purchased Acetylcholiesterase from Electrophus electricus (electric eel), C3389-2KU, following details are written on the enzyme vial. Type-VI S lyophilized powder 200-1000 Units/mg...

31 May 2024 6,444 1 View

What to do when GST- tagged protein is getting precipitated during dialysis?

I purified my recombinant GST-tagged protein in Tris buffer at pH 7.6 (the protein’s PI is 7.11) using a 50 mM reduced glutathione concentration in the elution buffer. However, after dialysis to...

21 May 2024 9,951 2 View

Need a research topic related to GST?

I need a research topic related to recent trends in GST

16 April 2024 1,805 0 View

How to expression and purify full length TRAF2 protein from bacteria?

Hi, I am for the first time struggling with TRAF2 full length protein purification from BL21 or Gold but I fail to purify it although I tried GST, MBP and His tag it the N- terminal. Do any...

07 April 2024 1,090 0 View

How to calculate (tot) glutathione peroxidase activity (GPx)?

Hi, I have been analyzing tot glutathione peroxidase (GPx) activity in mussel cells using cumene hydroperoxide (microplates) and I am a bit unsure how to read/calculate the results. I run a...

03 April 2024 9,344 0 View

Should I use the supernatant or lysate part of the cell?

When evaluating the expression of superoxide dismutase, catalase and glutathione peroxidase enzymes by ELISA in cell culture studies, should I use the supernatant or lysate part of the...

24 March 2024 5,247 2 View

How to measure electric conductivity (EC) in agricultural soil?

For Electric conductivity (EC), I have taken 10 g of soil in 25 ml of water and directly measured it without filtering the suspension. Is this correct ?

22 March 2024 2,351 3 View

Lalita Singh

here am attaching a file of my time scan readings..

Sadiq S Mareai

these papers will clarify your doubts..

Abdelrahim Ahmad Hunaiti

Unit/ml GST =A340/min test sample_A340 blank/min X 3 ml X dilution factor X9.6 Extinction coeffient X 0.1 ml enzyme volume

for more you can see our work in RG