total rxn mixture is 3 ml, 0.1 ml enzyme volume , 9.6mM is EC how to calculate the GST unit / min/ml in FW?
here am attaching a file of my time scan readings..
these papers will clarify your doubts..
Unit/ml GST =A340/min test sample_A340 blank/min X 3 ml X dilution factor X9.6 Extinction coeffient X 0.1 ml enzyme volume
for more you can see our work in RG
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