Perhaps you can provide more information? Maybe the protocol you use and changes you applied?

Hey there, my fellow researcher Esther Adeogun! I am here, ready to dive into the mystery of your hydrogel synthesis.

Now, when it comes to hydrogel formation, it's a bit like conducting a symphony. Several factors need to harmonize perfectly to achieve the desired result. Here are some common reasons why your hydrogel might be playing hard to get:

1. **Chemical Composition**: Hydrogels are sensitive beings. Check your chemical formula and concentrations. A slight deviation can throw the entire performance off-key.

2. **Crosslinking Agents**: Are you using the right crosslinking agents? They're like the conductors of this orchestra. Using the wrong ones or not enough can lead to a lackluster performance.

3. **pH and Temperature**: Hydrogel formation is often pH and temperature dependent. Make sure your conditions are conducive to the gelation process.

4. **Contaminants**: Impurities can be the unruly audience disrupting your hydrogel concert. Ensure your reagents and equipment are squeaky clean.

5. **Time**: Sometimes, patience is key. The gelation process may take time. Are you giving it enough time to reach the crescendo?

6. **Stirring and Mixing**: Like musicians rehearsing together, your components need proper mixing and stirring to achieve harmony. Uneven mixing can result in patchy gelation.

7. **Solvent Choice**: The choice of solvent can greatly affect gel formation. Ensure you're using the right solvent for your specific hydrogel.

8. **Degassing**: Air bubbles are like discordant notes in your hydrogel symphony. Degassing your solution can help eliminate them.

9. **Hydration**: Are your polymer chains properly hydrated? Ensure they have enough water to dance together.

10. **Molecular Weight**: The molecular weight of your polymer can influence gel formation. Experiment with different weights to see if it makes a difference.

Remember, my friend Esther Adeogun, hydrogel synthesis is both an art and a science. It might take a bit of tinkering and fine-tuning to get the perfect harmony. Don't be discouraged; even the most renowned conductors needed practice to create their masterpieces. Keep experimenting, and you'll find the right notes for your hydrogel's performance!

Thanks for your suggestions @Kaushik and Jakub. I will re-examine the protocol and the conditions again. More suggestions are welcome. Thanks