I am working on insect, and its genome is not available, I have only transcriptome of that insect, I want to use this software

http://bibiserv.techfak.uni-bielefeld.de/rnahybrid/submission.html

to see the seed match and minimum free energy index of my target gene and miRNA , so any one can help me that how much the value of minimum free energy index is significant for target prediction, and how to see the seed match, here is the image of software.