I have a gene of interest (assume X) which plays role in many developmental events in plants. But I am trying to find its role in abiotic stress sepcifically drought stress. I have checked the expression of X in few different tissues where X express abundantly under drought stress (data available in database and reports) which is significant.
I was thinking to explore how X gene controls the photosystem when the plants are under stress. X gene is already reported to be involved in the regulation of photosynthetic genes using trangenics and other interaction assays.
Now, I am stucked in what way I can proceed. Could anyone provide me any suggestions?
Hi Gayathri. Probably the way for most researchers would be working with transformed plants for the x gene (overexpressing, KO or RNAi, for example). This way you could treat the x transformed plants with drought conditions and evaluate photosynthesis. Specifically for photosystem parameters you may use a Dual-PAM or at least an equipament capable of measuring fluorescence (for PSII, in this case). Good luck!
Edit: You don't need to transform plants, by the way. There are lots of Arabidopsis mutants available (https://abrc.osu.edu), probably including ones related to your interest gene.
Dear,
You said:
-you got results, and
- that previous researchers have reported the involvement and role of your gene X in controlling drought stress.
So, I suggest to study its role in other stresses like salinity, temperature, chemical, and other stresses.
Or to study the factors that might affect its role in drought stress.
Greetings.
Assist. Prof. Dr. Murtadha H. Fayadh
Plant Physiologist, Basra, Iraq
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