Gayathri Kks

6 Questions 13 Answers 0 Followers

Questions related from Gayathri Kks

How can I perform in-silico analysis to identify the possible targets of my gene of interest (transcription factor) in arabidopsis and rice?

Using Genevestigator, we identified a set of genes co-expressing with my gene of interest (transcription factor). Now, I am using PlantPan database to check whether the co-expressed genes'...

15 March 2019 4,672 4 View

How to give drought and salt stress in soil for arabidopsis and tomato?

I am working in drought and salt stress with Arabidopsis and Tomato. I need to give these stress conditions to minimum 2 week old seedling. Could I give these stress treatment in soil by giving...

19 February 2019 8,490 14 View

How to get rid of non- specific band?

I am trying to amplify a gene using genomic DNA and Taq polymerase. I got two clear bands, one is the specific band with very low intensity and another band which is non-specific with very high...

27 July 2018 3,878 4 View

I need to amplify a full length gene using gene specific primers. How can I optimise the PCR conditions?

I'm using gradient PCR with Dream Taq polymerase enzyme and mentioned PCR conditions and components in protocol. I'm getting a high intensity non- specific band lower than the expected amplicon...

23 March 2018 4,812 8 View

In the preparation of 50X TAE buffer, when should we add glacial acetic acid? BEFORE or AFTER autoclaving the buffer ?

22 January 2018 6,084 3 View

I am working in drought stress in rice. Need some suggestions.

I have a gene of interest (assume X) which plays role in many developmental events in plants. But I am trying to find its role in abiotic stress sepcifically drought stress. I have checked the...

01 January 1970 7,582 4 View