12 December 2014 2 9K Report

I was wondering among other possibilities if maybe I should use a restriction enzyme to shear the bacterial DNA into smaller pieces.

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Do I need a restriction enzyme in order to sequence circular dna samples?

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Can sonication break DNA/oligonucleotides?

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Which is the best procedure for hybridizing two DNA strands in solution?

I have two DNA strands (one has 39 base pairs and the other 10 base pairs, both being complementary) and I want them to hybridize together in solution. I usually mix the two strands (final volum...

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