Antoni Llopis Lorente

5 Questions 5 Answers 0 Followers

Questions related from Antoni Llopis Lorente

Can sonication break DNA/oligonucleotides?

I have oligonucleotides attached to nanoparticles, and sometimes I need to sonicate the nanoparticles in order to redisperse them. Now, I am affraid that I could be breaking the DNA strands doing...

12 December 2014 1,791 3 View

I am using a DNA probe able to detect its complementary strand, but I am not able to detect bacterial DNA. What are the possible reasons for this?

I was wondering among other possibilities if maybe I should use a restriction enzyme to shear the bacterial DNA into smaller pieces.

12 December 2014 8,885 2 View

Do I need a restriction enzyme in order to sequence circular dna samples?

I got genomic dna samples and I wonder if need to break the circular strand in order to sequence it. How can I break it? And how can i know if I have circular or linear dna in my samples? Thanks a...

12 December 2014 473 8 View

Can I know if my dna samples are linear or circular by UV-vis spectroscopy?

I got genomic dna samples and I would like to know if it is in linear or circular form. Thanks.

12 December 2014 8,165 5 View

Which is the best procedure for hybridizing two DNA strands in solution?

I have two DNA strands (one has 39 base pairs and the other 10 base pairs, both being complementary) and I want them to hybridize together in solution. I usually mix the two strands (final volum...

11 November 2014 4,807 5 View