Dear Deepak, you may use FBS for culturing cells and starve confuent cells in serum free and antibiotic free basal medium for overnight before going for luciferase assay.

Thanks sir I want to know that does addition of other growth factors are just to support growth? and without them what are the major changes occur?

Hello Deepak

For HUVEC cells you need F-12K media + 0.1 mg/ml heparin + 0.03 to 0.05 mg/ml Endothelial cells growth supplement (ECGS) + 10% FBS

I hope that's answers your question and good luck